- Mississauga is serviced by seven major highways
- Highway 401 is a limited access multiple lane highway running from Windsor on the west to the Quebec border on the east. Often called "Ontario's Main Street", it is by far the busiest highway in Canada and North America. Its width varies and through the Greater Toronto corridor it is a complex system of express lanes, collector lanes and ramps. Highway 401 passes through Mississauga with six interchanges within the City.
- The Queen Elizabeth Way (QEW), Canada's first highway, connects the Greater Toronto Area with the international border at Niagara. The QEW provides entry to the U.S.A. at Lewiston, Niagara Falls and Buffalo and passes through Mississauga en route to downtown Toronto.
- Highway 403 is a multiple lane highway which connects the QEW with Highway 401 and provides an east/west highway link through the centre of Mississauga. There is a convenient interchange at Hurontario St. (Highway 10) with access to the Mississauga City Centre: a major concentration of mixed uses including office, residential, retail and entertainment. Carpool lanes (HOV - High Occupancy Vehicle lanes) are operational on Hwy. 403 allowing vehicles carrying more than one passenger to cut travel times.
- Highway 409 provides a direct link from Highways 401 and 427 to Toronto Pearson International Airport.
- Highway 410 provides a link northwards from Highways 403 and 401 through the City of Mississauga to the City of Brampton. It services a major portion of Mississauga's industrial area west of Pearson International Airport.
- Highway 427 is a north/south highway system linking the City of Toronto with Mississauga and areas to the north.
